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  • Intracytoplasmic Sperm Injection in Frozen thawed oocytes

    ICSI, Frozen thawed oocyte Since its advent in 1992, ICSI has become used routinely in the vast majority of IVF units and has been found to be a safe and effective means of treating male factor infertility. ICSI has proven to a powerful tool in overcoming severe male factor cases which, prior to ICSI, treated [...]

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In-vitro maturation of mammalian oocyte by our trainee in our training center

 

IVF in patients with previous ectopic pregnancy ( pregnancy in Fallopian tube)

I saw lot of patients with secondary infertility who had ectopic pregnancy after their marriage.

It shows that :

1. They are fertile .

2. The sperms and eggs are usually of good quality.

3. The fertilization site , that  is fallopian tube is at fault.  The tube is patent, ,it allows sperm to go to the egg ,that is waiting at the outer portion of the fallopian tube. The sperm fertilizes the egg, but when the tube pushes the fertilized egg to the uterus , it gets trapped in the tube. It happens because the internal surface of the tube is not smooth. The uterus grows with the growing embryo , but tube can not, so it cracks and the patients land into the severe internal bleeding ( if not seen earlier part of pregnancy). Usually she gets operated and the tube with the pregnancy is removed. Now the lady is with only one tube left.

4. Now she tries for another pregnancy, but couldn’t achieve it. Why? ,as she is fertile, her periods are regular, ,she produces eggs in every month, and staying with her husband, but without pregnancy. This happens because : She had a pregnancy ( ectopic) with the better tube she had ( and it is removed, as she had ectopic). The tube left is usually inferior compared to the tube that is removed, Otherwise she could have a normal pregnancy with this remaining tube.

5. Now the treatment starts , and a Hysteros Salpingo graphy ( HSG) / laparoscopy is done to see the remaining tube , and report comes : patent tubes. Now she is confused . If the remaning tube is patent, then she should have  a natural pregnancy. On that quest : She undergoes lots of useless treatments, like multiple Intra-uterine inseminations (IUI). If her remaining tube is not good , then what ever you do ( with putting sperm in her uterus,IUI) fertilization does not happen.

6. This is because : Tube has to do three functions: a. give passage to sperms and eggs, b. Give nutrition to the fertilized eggs, c. Propel the fertilized egg( embryo) to the uterine cavity. Fault in any of the three function will hamper pregnancy in uterine cavity.

7. So the treatment is not putting sperms in uerince cavity (IUI) , as IUI has not created her earlier ectopic, The treatment is Fertilization. If it is not happening in tube ( in-vivo) , then she should have fertilization , In-vitro ,that is IVF

8. I see many patients with previous ectopic , aho waste their time and money in these useless treatments. You can earn money but you can not recover the age. Age makes the eggs poor, and when they come late , the chances of IVF gets poorer. They are increasing their problems and reducing the chances of getting pregnancy because of their own ( ? treating physician’s) ignorance.

9. If they come early for IVF , the chances of success will be more as compared to primary infertility patients ( who never has pregnancy).

10. In India , the tubes are damaged due to Tuberculosis infection(?). some times this inffection damages the uterine lining also ( endometrium).

11. So more delay in getting IVF; May create problem in both ” Seed and the Soil”

Some happenings in Ideal Fertility

 

 

 

 

3-D scan of uterine cavity, training session in embryology lab, embryology lab, 3-D imaging of IVF fetus

 

3-D uterine cavitytraining in Embryology labEmbryology lab3-D image of an IVF fetus

blastocyst transfer

Blastocyts developed in ASTEC incubatorujjawal 5.3 good

Testicular Sperm extraction and Frozen Embryo transfer

This case is from an Azoospermia ( Nil sperms) husband (married life 12 yrs). we did : Testicular sperm extraction and In-vitro fertilization. Three blastocysts ( human embryos) developed on day 5, but on that day wife started bleeding and we do not transfer them and vitrified ( freeze ) them .. We did Frozen embryo transfer in next (down regulated) cycle with hormone replacement. She conceived and today we did ultrasound and found that she has live twin pregnancy. T.

Bridge course ( Biotech) : University to industry

Study Between Conventional and Benchtop Incubators

Reasons behind liking towards Benchtops ( with gas mixers ) 1. Its recovery time is fast. 2. You can adjust the gas concentrations according to your need ( what you can not do with simple warmer like benchtops). 3.Its a direct heat transfer technology,so regaining the temperature is fast. 4. Upper lid is also warmed and slightly higher than the base ( so evaporation is is less hence change in osmolality of media in long term culture will be less,( hence no need of waterbody, maybe similar to embryoscope,which I don’t have. 5. Multiple individual chambers for each patient ( we can keep them exclusively for one patient). 6. Occupies less space in lab. We get hatching blasts in my Benchtop, we get pregnancies too,but compared to blast transfers of both system, conventional gave better pregnancy rates . Questions is :Can we improve it more or similar to conventional,. We are doing this study from pre-equilibration to day5 with sibling oocytes in both systems, with same gas concentrations ( CO2 concentration is set according to the pH measured in conventional one). I am trying to get ideas from my fellow IVF professionals and embryomailers ( in other forum). I recieved a beautiful paper on various incubation system ,(but still less with benchtops with gas mixers), which I can share on request.

If you are coming to us : Read this website, It will help you.

One of my French patient, written this documentary, It will help any body to know the details and ambiance of the city and our way of working

http://isabellestroh-babydesire.123siteweb.fr

One of the best IVF clinic in world

A patient with severe hirsutism and PCOD. Under gone thrice IVF, first two cancelled because of ,first no stimulation and then hyperstimulation. Thiird attempt: stimulation for 6 days and Hcg when follicles are small. one egg retrieved…In-vitro matured for two days then ICSI ied ..Led to Pregnancy, Developed severe diabetes in early pregnancy ,partially controlled by Insulin,but aborted at 20 weeks.
Now she is again referred from Nagpur,Fourth attempt…no eggs
Fifth attempt : stimulation for 7 days ( FSH and GnRhanta). then hCG…three immature eggs ( see vedio) ,.In-Vitro matured for two days…Reached MII stage..ICSI ied by Dr. Rinku Banerji , ….Embryos transfered on day 3 ( See Video)….Waiting for the result
immature eggs of Sumedha Chaubeysumedha 27.2_xvid

Hatching Blastocyst

hatching blast,inner cell mass, 1.3.2014


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