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  • Intracytoplasmic Sperm Injection in Frozen thawed oocytes

    ICSI, Frozen thawed oocyte Since its advent in 1992, ICSI has become used routinely in the vast majority of IVF units and has been found to be a safe and effective means of treating male factor infertility. ICSI has proven to a powerful tool in overcoming severe male factor cases which, prior to ICSI, treated [...]


Be Careful!


I would like to share this information with everybody who carries out KARYOTYPING in their diagnostic laboratory.  We changed our routine protocol of Lymphocyte Culture after reading the manual of a newly purchased Culture Media (I would not like to mention the name of the company), which assured the increase in the number of metaphases if the colchicine incubation was increased. We followed the same (made it 1hr) and did observe the change. We got better and more fields but….. that was an invitation to another problem.

Yes and by the time we realised it was almost close to a month struggling to get crisp chromosome banding. We thought of a lot of possibilities like trypsin not getting activated, change in pH of NS used, change in the buffer concentration, over aging of slides, mishandling, etc. After trying various other protocols for G banding and not getting good results, we traced back the changes we made in our routine protocol. And then again colchicine came into picture. After bringing incubation time back to 15mins we again got banded chromosomes with basic G Banding protocol.

So please DO NOT blindly follow the text mentioned in the manuals. It might bring about the change they promise but somewhere CHANGE your results as well!!!


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