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  • Intracytoplasmic Sperm Injection in Frozen thawed oocytes

    ICSI, Frozen thawed oocyte Since its advent in 1992, ICSI has become used routinely in the vast majority of IVF units and has been found to be a safe and effective means of treating male factor infertility. ICSI has proven to a powerful tool in overcoming severe male factor cases which, prior to ICSI, treated [...]

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Each Bad Day Is a Step towards Learn Something New

Roopsi Bhatia M.Sc. Molecular Biologist
In order to analyze chromosomes we follow a set procedure in our Molecular Diagnostics Lab, i.e. Lymphocyte Culture  Slide Preparation  Trypsin Treatment  G-Banding. I have been following the same for quite a long time and this standard protocol gave wonderful results and was able to give reports within 5 days.
Problem started when there was a change in the season and mercury dropped! This somehow did change the nature of trypsin and it didn’t get activated properly though I tried all the possible ways to provide the right temperature conditions :-/ So at the end of the day I saw only nonbanded metaphases and to be mentioned they were really clear but still didn’t make really happy.
When my mentor, Dr. Mrs. Rinku Banerji, caught this state of mine she asked me to read “The AGT Cytogenetics Laboratory Manual” and this cleared all my doubts. It was well mentioned in the manual – “chromosome morphology is more easily seen on NONBANDED preparations, particularly the terminal ends of chromosomes, which tend to become unclear with trypsin banding.” After reading the entire column I felt that it can be helpful to look at nonbanded metaphases and I did the same. I could check metaphases for numeric and morphologic abnormalities.
My day ended taking chromosomes photographs and preparing karyotype, teaching me a lesson no matter how skilled we are BOOKS and BOSS will always keep feeding you with something new 


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